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bone marrow derived macrophages bmdms  (Charles River Laboratories)

 
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    Charles River Laboratories bone marrow derived macrophages bmdms
    Bone Marrow Derived Macrophages Bmdms, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdms/bone+cells+marrow/pmc08738146-49-2-15
    Average 86 stars, based on 1 article reviews
    bone marrow derived macrophages bmdms - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Lysis:

    Article Title: Tissue-Specific Targeting of Inflammatory Pathways in Osteoarthritis
    Article Snippet: Methods BMDMs were han/ested from the femurs of3 month old Lewis raB (Charles River Laboratories). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA).

    Modification:

    Article Title: Tissue-Specific Targeting of Inflammatory Pathways in Osteoarthritis
    Article Snippet: Methods BMDMs were han/ested from the femurs of3 month old Lewis raB (Charles River Laboratories). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA).

    Recombinant:

    Article Title: Tissue-Specific Targeting of Inflammatory Pathways in Osteoarthritis
    Article Snippet: Methods BMDMs were han/ested from the femurs of3 month old Lewis raB (Charles River Laboratories). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA). .. To isolate and differentiate BMDMs, bone marrow was flushed out of the femurs and the cells were treated with ACK lysis buffer (lnvitrogen), centrifuged, and resuspended in D-1O media, which consists of Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 110m mg/L sodium pyruvate, 1% penicillin-streptomycin, 10% heat-inactivated FBS, and 25 ng/mL recombinant rat macrophage colony stimulating factor (M-CSF) (BioLegend, San Diego, CA).

    Generated:

    Article Title: c-Myc inhibits macrophage antimycobacterial response in Mycobacterium tuberculosis infection.
    Article Snippet: .. BMDMs were generated from C57BL/6J female mice (Charles River Laboratories). ..

    Transgenic Assay:

    Article Title: Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells
    Article Snippet: Monocytes were differentiated using 100 nM phorbol 12-myristate 13-acetate (Sigma, P1585-1 MG) treatment for 72 h. Foam cells were generated by incubation with agLDL (50 μg/mL) for 30 h followed by an equilibration period of 24 h with or without 10 μM chloroquine (Sigma, C6628) in RPMI 1640 medium supplemented with 2 mg/mL fatty acid-free bovine serum albumin (BSA; Sigma, A6003). .. BMDMs were flushed from the femurs of C57BL/6 N (Charles River Laboratories, 027) or GFP-LC3 transgenic mice (Riken, RBRC00806) and differentiated to macrophages by incubation in DMEM medium (Corning, 10013 CV) supplemented with 10% FBS, 1% P/S, and 20% L929-conditioned medium for 7 days. .. Peritoneal macrophages were harvested 4 days after intra-peritoneal injection of 3% thioglycolate (BD Difco, B243010), as previously described [ ] and cultured in DMEM media supplemented with 10% FBS and 1% P/S.

    Incubation:

    Article Title: Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells
    Article Snippet: Monocytes were differentiated using 100 nM phorbol 12-myristate 13-acetate (Sigma, P1585-1 MG) treatment for 72 h. Foam cells were generated by incubation with agLDL (50 μg/mL) for 30 h followed by an equilibration period of 24 h with or without 10 μM chloroquine (Sigma, C6628) in RPMI 1640 medium supplemented with 2 mg/mL fatty acid-free bovine serum albumin (BSA; Sigma, A6003). .. BMDMs were flushed from the femurs of C57BL/6 N (Charles River Laboratories, 027) or GFP-LC3 transgenic mice (Riken, RBRC00806) and differentiated to macrophages by incubation in DMEM medium (Corning, 10013 CV) supplemented with 10% FBS, 1% P/S, and 20% L929-conditioned medium for 7 days. .. Peritoneal macrophages were harvested 4 days after intra-peritoneal injection of 3% thioglycolate (BD Difco, B243010), as previously described [ ] and cultured in DMEM media supplemented with 10% FBS and 1% P/S.

    Nitric Oxide Assay:

    Article Title: Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis.
    Article Snippet: .. Colorimetric nitric oxide assay BMDMs were isolated from 3 to 4 months old 129S2 mice (Charles River Laboratories, Wilmington, Massachusetts), IFNγ-activated, infected with Mtb H37Rv and treated as described in the “Bonemarrow-derived macrophage infection studies” section. ..

    Isolation:

    Article Title: Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis.
    Article Snippet: .. Colorimetric nitric oxide assay BMDMs were isolated from 3 to 4 months old 129S2 mice (Charles River Laboratories, Wilmington, Massachusetts), IFNγ-activated, infected with Mtb H37Rv and treated as described in the “Bonemarrow-derived macrophage infection studies” section. ..

    Infection:

    Article Title: Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosis.
    Article Snippet: .. Colorimetric nitric oxide assay BMDMs were isolated from 3 to 4 months old 129S2 mice (Charles River Laboratories, Wilmington, Massachusetts), IFNγ-activated, infected with Mtb H37Rv and treated as described in the “Bonemarrow-derived macrophage infection studies” section. ..



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    Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

    Journal:

    Article Title:

    doi:

    Figure Lengend Snippet: Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

    Article Snippet: Where indicated, BMDM monocultures were activated with 10 ng/mL lipopolysaccharide (LPS; tlrl-3pelps, Invivogen) and 10 ng/mL murine interferon gamma (IFNγ; Abcam), or 10 ng/mL murine interleukin (IL-4; PeproTech) and 10 ng/mL IL-13 (PeproTech), as indicated.

    Techniques: Co-culture Assay, Expressing, Cell Culture, Flow Cytometry, Analysis, Fluorescence